Day :
Keynote Forum
Prof. Dr. Şükrü Tüzmen
1Professor, Eastern Mediterranean University (EMU), Famagusta, T.R.N.C., Mersin 10 – Turkey
Keynote: RNAi-based personalized therapeutic strategies in Oral Cancers: How far have we come?
Time : 11:00 - 11:30
Biography:
Prof. Dr. Åžükrü Tüzmen has completed his PhD at the age of 31 from Bosphorus University, Istanbul, and six-year postdoctoral studies at the National Institutes of Health (NIH), USA as a Fogarty Fellow, and as the first Turkish Cypriot scientist to work on globin gene regulation at the NIH.
His mission is to discover and validate links between gene states and disease phenotypes, and further use these links to identify druggable targets to be utilized as biomarkers in the early diagnosis stages of genetic diseases such as cancer.
He has published numerous papers in reputed journals and has been serving as an editorial board member of repute.
Abstract:
A classical technique to determine the function of a gene is to experimentally inhibit its gene expression in order to examine the resulting phenotype or effect on molecular endpoints and signaling pathways.
RNA interference (RNAi) is one of the recent discoveries of a naturally occurring mechanism of gene regulation facilitated by the induction of double stranded RNA into a cell. Synthetic short interfering RNAs (siRNAs) can be designed to silence the expression of specific genes bearing a particular target sequence and may potentially be presented as a therapeutic strategy for inhibiting transcriptional regulation of genes, which in such instances constitute a more attractive strategy than small molecule drugs. Commercially available RNAi libraries have made high-throughput genome- scale screening a feasible methodology for studying complex mammalian cell systems. However, it is crucial that any observed phenotypic change be confirmed at either the mRNA and/or protein level to determine the validity of the targeted genes.
Here, we describe a high-throughput screening of RNAi based gene knock-down approach and qPCR validation of specific transcript levels. Oral cancers are most often discovered after they have spread to the lymph nodes of the neck. Early detection is key to surviving oral cancer.
In light of such advantageous applications, siRNA technology has become an ideal research tool for studying gene function in various research fields, and holds the promise that the utilization of siRNA- based therapeutic agents will accelerate drug discovery in clinical trials including oral cancers.
Session Introduction
Hikmet SOLAK
Eastern Mediterranean University Faculty of Dentistry
Title: The Effectiveness Of Using Digital Scanning Method For Reducing Iatrogenic Damage To The Adjent Teeth Surfaces During Class II Cavity Preparations
Biography:
Hikmet SOLAK has completed his PhD in Ankara University, Faculty of Dentisty in 1985 He became Professor in 2002. In 2007 he joined Near East University as a Founder of Faculty of Dentistry. In 2019 he joined to Eastern Mediterranean University Faculty . He currently working as Professor of Restorative Dentistry Department in Eastern Mediterranean University He has various published more than 60 papers in reputed journals and has been serving as an Chief editorial board member of repute. He is also Chief and member of Turkish Fishing Organisation, and member of Turkish Dental Tourism Organization
Abstract:
To evaluate the effectiveness of digital scanning methods in dental education to reduce the rate of damage to the adjent teeth in CL II cavity preparations Materials and Methods: As a part of cavity preparation lecture ; after teaching them how to open cavities without any deformation to the adjent teeth ; 245 Dental students were asked to prepare classical Class II cavity preparations both in maxiller and mandibular first molars in phantom models. Mesio-occlusal class II cavities for maxiller first molar-16 were performed and disto-occlusal Cl II cavities performed for mandibular 1 molar-36. All adjent phantom teeth surfaces were examined using visual examination then randomly 50 phantom models were selected and all adjent phantom teeth surfaces of that selected group were examined using 3D digital scanning methods. Examination of teeth surfaces were done by calibrated individual examiners in all groups. After examination, 50 selected performance owners were informed about their Iatrogenic damages. All 245 dental students were taken under a course again to teach them how to reduce iatrogenic damages for class II cavity preparations. The same procedures were repeated and students were asked to do same the work with the same teeth. Damages were scored as 0= no damage, 1= abrasion 2= injury <50% of surface, 3= injury > 50% of surface. Results: A rate of nearly 27 % less damage was found to the adjent teeth with the group that was shown their mistakes by digital scanning methods. After 2 courses, we still obtained iatrogenic damages Conclusion: According to our results of damage to the adjent teeth in CL II cavity preparations, additional teaching of protection of these surfaces is needed to reduce the rate. Also, the use of 3D scanning methods seems to be helpfull for us to obtain more successful results from cavity preparation teaching without the iatrogenic damages of adjent teeth.
Hikmet SOLAK
Eastern Mediterranean University Faculty of Dentistry, Mersin10
Title: The Effectiveness Of Using Digital Scanning Method For Reducing Iatrogenic Damage To The Adjent Teeth Surfaces During Class II Cavity Preparations
Biography:
Hikmet SOLAK has completed his PhD in Ankara University, Faculty of Dentisty in 1985 He became Professor in 2002. In 2007 he joined Near East University as a Founder of Faculty of Dentistry. In 2019 he joined to Eastern Mediterranean University Faculty . He currently working as Professor of Restorative Dentistry Department in Eastern Mediterranean University He has various published more than 60 papers in reputed journals and has been serving as an Chief editorial board member of repute. He is also Chief and member of Turkish Fishing Organisation, and member of Turkish Dental Tourism Organization
Abstract:
To evaluate the effectiveness of digital scanning methods in dental education to reduce the rate of damage to the adjent teeth in CL II cavity preparations Materials and Methods: As a part of cavity preparation lecture ; after teaching them how to open cavities without any deformation to the adjent teeth ; 245 Dental students were asked to prepare classical Class II cavity preparations both in maxiller and mandibular first molars in phantom models. Mesio-occlusal class II cavities for maxiller first molar-16 were performed and disto-occlusal Cl II cavities performed for mandibular 1 molar-36. All adjent phantom teeth surfaces were examined using visual examination then randomly 50 phantom models were selected and all adjent phantom teeth surfaces of that selected group were examined using 3D digital scanning methods. Examination of teeth surfaces were done by calibrated individual examiners in all groups. After examination, 50 selected performance owners were informed about their Iatrogenic damages. All 245 dental students were taken under a course again to teach them how to reduce iatrogenic damages for class II cavity preparations. The same procedures were repeated and students were asked to do same the work with the same teeth. Damages were scored as 0= no damage, 1= abrasion 2= injury <50% of surface, 3= injury > 50% of surface. Results: A rate of nearly 27 % less damage was found to the adjent teeth with the group that was shown their mistakes by digital scanning methods. After 2 courses, we still obtained iatrogenic damages Conclusion: According to our results of damage to the adjent teeth in CL II cavity preparations, additional teaching of protection of these surfaces is needed to reduce the rate. Also, the use of 3D scanning methods seems to be helpfull for us to obtain more successful results from cavity preparation teaching without the iatrogenic damages of adjent teeth.
Prof. Dr. Şükrü Tüzmen
Professor, Eastern Mediterranean University (EMU), Famagusta, T.R.N.C., Mersin 10 – Turkey
Title: RNAi-based personalized therapeutic strategies in Oral Cancers: How far have we come?
Biography:
Prof. Dr. Åžükrü Tüzmen has completed his PhD at the age of 31 from Bosphorus University, Istanbul, and six-year postdoctoral studies at the National Institutes of Health (NIH), USA as a Fogarty Fellow, and as the first Turkish Cypriot scientist to work on globin gene regulation at the NIH.
His mission is to discover and validate links between gene states and disease phenotypes, and further use these links to identify druggable targets to be utilized as biomarkers in the early diagnosis stages of genetic diseases such as cancer.
He has published numerous papers in reputed journals and has been serving as an editorial board member of repute.
Abstract:
A classical technique to determine the function of a gene is to experimentally inhibit its gene expression in order to examine the resulting phenotype or effect on molecular endpoints and signaling pathways.
RNA interference (RNAi) is one of the recent discoveries of a naturally occurring mechanism of gene regulation facilitated by the induction of double stranded RNA into a cell. Synthetic short interfering RNAs (siRNAs) can be designed to silence the expression of specific genes bearing a particular target sequence and may potentially be presented as a therapeutic strategy for inhibiting transcriptional regulation of genes, which in such instances constitute a more attractive strategy than small molecule drugs. Commercially available RNAi libraries have made high-throughput genome- scale screening a feasible methodology for studying complex mammalian cell systems. However, it is crucial that any observed phenotypic change be confirmed at either the mRNA and/or protein level to determine the validity of the targeted genes.
Here, we describe a high-throughput screening of RNAi based gene knock-down approach and qPCR validation of specific transcript levels. Oral cancers are most often discovered after they have spread to the lymph nodes of the neck. Early detection is key to surviving oral cancer.
In light of such advantageous applications, siRNA technology has become an ideal research tool for studying gene function in various research fields, and holds the promise that the utilization of siRNA- based therapeutic agents will accelerate drug discovery in clinical trials including oral cancers.