Dental Education

Biography

Biography: Farhat Kazmi

Abstract

Many carcinogenic agents are emphasized as predisposing factors for dysplastic changes leading to OSCC, but inflammation caused by odontogenic trauma has been ignored to certain extent. NF-κB family has vital role in the inflammation and is considered to be involved in the initiation and progression of oral cancer. The purpose of this study was to see the cause and effect relationship between the traumatic inflammation and the dysplastic changes observed in confirmed cases of OSCC using NF-κB pathway. The study was conducted on a sample of 28 patients of OSCC collected from University affiliated hospitals. Among these 23% patients presented with the clinical evidence of odontogenic trauma females made up 48% of cases, while 52% were males. The majority of lesions occurred on the buccal mucosa (57.14%) followed by tongue (33.33%) and retromolar region (9.53%). These specimens were then processed for the NF-κB analysis. The real time PCR reactions were set up for each sample after reverse transcriptase (RT-PCR) reactions with Eva Green primers. All the patients presented with mucosal trauma due to odontogenic reason showed threefold increase of NF-κB amplification, as compared to the other OSCC samples suggesting that inflammation caused as a result of trauma was one of the important initiating factors for dysplastic changes. Early detection and elimination of local trauma due to odontogenic causes could have prevented the dysplastic changes.